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CFU Calculator

Enter the number of colonies and dilution factor and volume of the cultural plate and the tool will find the CFU.

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CFU Calculator

The CFU Calculator calculates the total number of bacteria per unit weight or volume. The CFU stands for the Colony Forming Unit CFU/ml and it is essential to estimate the number of colonies by the CFU/ml calculator in a unit area.

What Is CFU Counting?

CFU stands for 'colony forming unit'. A bacterial concentration is a measurement of the number of colonies of bacteria per unit ml volume of the culture plate.

The total count of observable colonies that are there on an agar plate can be multiplied by the dilution factor for providing the resulting CFU/ml.

CFU/ml Formula:

A CFU is a colony-forming unit and describes the popularity of the microorganism concentration as a test sample. The agar plate concentration is essential to grow a healthy colony of the microorganism.

The CFU ml calculation formula is given:

CFU = (nc)(Df) Vc

Where:

  • nc = Number of colonies
  • DF = Dilution Factor 
  • Vc = Volume of the culture plate 

The number of colonies per unit volume of the cultural plate can be calculated by the CFU calculator to grow a healthy microbes colony. It would assist grow the specific colonies in the given concentration of the chemical.

How to Calculate CFU?

Suppose that you need to grow 9 colonies and the dilation factor is 7 and the volume of the 10 m^3. Then cfu calculation for a given solution is given by:

Given:

  • No. of Colonies= 9
  • Dilution Factor [DF]  = 7
  • Volume of Culture Plate= 10 m³

Solution:

CFU = (nc)(Df) Vc

CFU = (9)(7) 10

CFU = 63 10 

CFU  = 6.3 CFU/mL

The CFU g calculation is simple to convert as 10g is equal to 10 ml, and so on.

How this CFU Calculator Works?

The straightforward CFU/ml calculator is functioning. A couple of steps are given below:

Input:

  • Enter the number of colonies, Dilution factor, and volume of the culture plate
  • Tap the "CALCULATE" button

Output:

  • Colony Forming Unit (CFU)
  • Detailed measurements of CFU

FAQ

How Many Cells Are in 1 CFU?

The number of organisms in a single visible colony contains approximately one billion cells (1 x 109 or log 9 cells). However, 1 CFU (Colony Forming Unit) does not represent an exact number of cells because one colony may grow from a single live cell or a cluster of cells on an agar plate.

References:

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